Propogation of seaweed in Enrichment Media

Propagation of Seaweed in Enrichment Media

The species use in this experiment was Gracilaria changii. Three different media was use with three replicates. For our group, media that we used was B13, B14, B15 and B16.

Methodology

1.      Wash samples using tap water.

2.      Cut nine samples with measurement of 1cm.

3.      Weigh the samples one by one.

4.      Wash all samples with sterile water for about 30 second.

5.      Fill well plate with sterile seawater. (5ml)

6.      Add 10⁴ CFU/ml of selected media (bacteria) to three samples with three replicates.

7.      Put samples in each well.

8.      Observation was done for 7 days.

Picture 1 :   Cut nine samples with measurement of 1cm.

Picture 2 : Wash all samples with sterile water for about 30 second.

Picture 3 : Put each sample inside the well with filled of water.

Picture 4 : Put 10⁴ CFU/ml of selected media (bacteria) to three samples with three replicates.

Picture 5 : Three replicate of sample with bacteria as the media

Picture 6 : The weight of each sample

Picture 7 : The cuting seaweed in each well with sterile water

Result

Picture 8 : Some of the cutting show some bud

Discussion

Based on the result, some cutting of sample shows the growth of bud at the thallus. This shows that the thallus of sample had positive effect to interact with the bacteria media gives the rapidly grow of the thallus. However, some cutting of sample didn't show any reaction this is cause by the media which is contaminated. It slow the bacteria activity for the thallus of sample. Thus, the active bacteria will give  a positive effect to the growth of the seaweed even though it takes time but the effect of growth is low if the bacteria media is damaged or contaminated.