Propogation of Aquatic Plant

Propogation of Aquatic Plant (Mesea  sp. )

Introduction

Propagation refers to the process of making more plants to keep a plant variety alive. Propagation is mainly done to improve plant health. Dividing a plant and replanting it stimulates new growth. Commonly, aquatic plants show two types of propagation. Vegetative propagation takes place when a part of the plant itself is used to propagate the plant.

Materials

Samples (Mesea sp), bottle with small screen covered on top, sterile distilled water, tween-20, Chlorox (70%, 50%, 30% and 10%), 70% ethanol, petri dish, Media ( Murashigae and Skoog agar), test tube.

Methodology

1.      The root and leaf was cut off from the plant.

2.      The plant is cut with length of 1cm each for 5 samples.

3.      The explants is washed with sterile distilled water with 2-3 drops of Tween-20 for 3 minutes

4.      The explants then been washed with 70%, 50%, 30% and 10% chlorox for 1 minute each.

5.      The explants then been rinsed with sterile distilled water.

6.      The explants is kept in the small bottle and brought to laminar flow.

7.      The explants then washed using 70% ethanol

8.      Put the explants on sterile tissue and cut the dead rhizomes

9.      Explants were planted into agar.

Picture 1 : Cutting the leaves or thallus of the sample

Picture 2 : The explants is washed with sterile distilled water with 2-3 drops of Tween-20 for 3 min

Picture 3 & 4 : The explants then been washed with 70%, 50%, 30% and 10% chlorox for 1 minute each.

Picture 5 : Cutting the dead rhizomes  inside laminar flow

Result :

Our sample had shown a negative result which is the plant didn't show any grow at a media inside a beaker.

Discussion :

Based on our result, all the cutting plant didn't grow any bud it because  plant is contaminate due to our inproper method during the cutting part inside the laminar flow . We didn't do it properly cause the bacteria to grow of the plant.  The contaminate plant also effect the media as well so the plant didn't receive any  benefits from media as a result both of them shows a negative affect.