PREPARATION OF MEDIA

Practical 1

PREPARATION OF MEDIA

Algae media culture is known as an artificial environment in which let the algae growth. To make sure the algae growth in artificial medium there are a few important aspects need to being looking at such as nutrient's quantity and quality, light, pH, turbulance, salinity and temperature. In this experiment we used Von Stosch Enrichment (VSE). The following are their methods:

1. Firstly we take 4 conical flasks.

2. Each of them pour 500 ml deionized water.

3. Put different solutions in each flasks which is nitrogen, phosphate, iron and EDTA respectively.

Solution 1 : Nitrogen

Pour 1L of deionized water and put 26.75g Ammonium chloride (NH4 CL)

Solution 2 : Phosphate

Pour 1L of deionized water and sodium phosphate. dibasic , 12-hydrate and crystal with weight os 0.4g .

Solution 3 : Iron* (combine with 4 immediately prior to addition to seawater)

Pour 1L of deionized water and put 0.28 of Ferrous sulphate (FeSo₄.7H₂O)

Solution 4 : EDTA* (combine with 3 immediately prior to addition to seawater)

Pour 1L of deionized water and 3.72g disodium ethylenediamine tetra acetate (Na₂EDTA)

4. After done putting the needed ingredients in each flasks , mixed them using a prepared machine.

5. Lastly, after done mixing, our 4 conical flasks combine with other 2 conical flasks that consists manganese and vitamins solutions.